Novel submicroscopic extrachromosomal elements containing amplified genes in human cells.

doi:10.1038/327434a0

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Article

Novel submicroscopic extrachromosomal elements containing amplified genes in human cells.

Nature - 1 Jan 2000

Maurer B J, Lai E, Hamkalo B A, Hood L, Attardi G

Abstract excerpt

In previous work, several methotrexate (MTX)-resistant variants were isolated frm the human cell line HeLa BU25, which exhibited a high degree of dihydrofolate (DHFR) gene amplification (estimated to be 250- to 300-fold). These variants did not contain any chromosome with a homogeneously staining...

Topics

Drug Resistance
Extrachromosomal Inheritance
Gene Amplification
Genes
Genetic Variation
HeLa Cells
Humans
Methotrexate
Tetrahydrofolate Dehydrogenase

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