Article
Early appearance and long-term persistence of the submicroscopic extrachromosomal elements (amplisomes) containing the amplified DHFR genes in human cell lines.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Pauletti G, Lai E, Attardi G
Abstract excerpt
Submicroscopic extrachromosomal elements (amplisomes) containing amplified dihydrofolate reductase (DHFR) genes have been investigated in a methotrexate-resistant derivative of the human cell line HeLa BU25, 10B3, by field-inversion gel electrophoresis. The amount and kinetics of formation of these elements have been correlated with the level and time course of overall DHFR gene amplification. The amplisomes...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.