Early appearance and long-term persistence of the submicroscopic extrachromosomal elements (amplisomes) containing the amplified DHFR genes in human cell lines.

doi:10.1073/pnas.87.8.2955

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Article

Early appearance and long-term persistence of the submicroscopic extrachromosomal elements (amplisomes) containing the amplified DHFR genes in human cell lines.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Pauletti G, Lai E, Attardi G

Abstract excerpt

Submicroscopic extrachromosomal elements (amplisomes) containing amplified dihydrofolate reductase (DHFR) genes have been investigated in a methotrexate-resistant derivative of the human cell line HeLa BU25, 10B3, by field-inversion gel electrophoresis. The amount and kinetics of formation of the...

Topics

Blotting, Southern
Cell Line
DNA, Neoplasm
Drug Resistance
Ethidium
Gamma Rays
Gene Amplification
Genes
Genetic Variation
HeLa Cells
Humans
Kinetics
Methotrexate
Tetrahydrofolate Dehydrogenase

Source

Open in PubMed Open full text

Identifiers

PMID: 2326258
DOI: 10.1073/pnas.87.8.2955
PMCID: PMC53812

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2326258-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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