Article
Generation of induced pluripotent stem cell line (RCMGi008-A) from human skin fibroblasts of a cystic fibrosis patient with compound heterozygous F508del/CFTRdele2.3 mutations in CFTR gene.
Stem cell research - 1 Aug 2022
Kondrateva Ekaterina, Panchuk Irina, Demchenko Anna, Grigorieva Olga, Zheglo Diana, Voronina Ekaterina, Erofeeva Anastasija, Tabakov Vyacheslav, Orlova Maria, Lavrov Alexander, Smirnikhina Svetlana, Kutsev Sergey
Abstract excerpt
Skin fibroblasts obtained from a 20-year-old woman with clinically manifested and genetically proven (F508del/CFTRdele2.3) cystic fibrosis were successfully transformed into induced pluripotent stem cells (iPSCs) by using Sendai virus-based reprogramming vectors including the four Yamanaka factors, OCT3/4, SOX2, KLF4, and c-MYC. The iPSCs showed a normal karyotype, expressed pluripotency markers and exhibited the...
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