Article
Generation of two induced pluripotent stem cell lines (RCMGi004-A and -B) from human skin fibroblasts of a cystic fibrosis patient with compound heterozygous F508del/W1282X mutations in CFTR gene.
Stem cell research - 1 Apr 2021
Kondrateva Ekaterina, Demchenko Anna, Slesarenko Yana, Pozhitnova Victoria, Yasinovsky Matvey, Amelina Elena, Tabakov Vyacheslav, Voronina Ekaterina, Lavrov Alexander, Smirnikhina Svetlana
Abstract excerpt
Skin fibroblasts obtained from a 28-year-old man with clinically manifested and genetically proven (F508del/W1282X) cystic fibrosis were successfully transformed into induced pluripotent stem cells (iPSCs) by using non-viral, non-integrating, self-replicating RNA reprogramming vectorthat contains five reprogramming factors: OCT4, KLF4, SOX2, GLIS1, and c-MYC as well as a puromycin-resistance gene. Two iPSC lines...
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