Article
Generation of two induced pluripotent stem cell lines (RCMGi005-A/B) from human skin fibroblasts of a cystic fibrosis patient with homozygous F508del mutation in CFTR gene.
Stem cell research - 1 Oct 2022
Panchuk Irina, Kondrateva Ekaterina, Demchenko Anna, Grigorieva Olga, Erofeeva Anastasija, Amelina Elena, Tabakov Vyacheslav, Orlova Maria, Voronina Ekaterina, Pozhitnova Victoria, Lavrov Alexander, Smirnikhina Svetlana, Kutsev Sergey
Abstract excerpt
Induced pluripotent stem cells (iPSCs) was successfully generated from skin fibroblast obtained from patient with cystic fibrosis by using non-integrating, viral CytoTune™-iPS 2.0 Sendai Reprogramming Kit, which contain three vectors preparation: polycistronic Klf4-Oct3/4-Sox2, cMyc, and Klf4. Created iPSC lines showed a normal karyotype, expressed pluripotency markers and demonstrated the potential to...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.