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Highly sensitive detection of EGFR L858R mutation at the mRNA level.

Analytical biochemistry - 1 Oct 2022

Pham Mai, Pham Quynh, Nguyen Ung, Nguyen Lanh, Nguyen Hoa, Vu Thang, Nguyen Ba, Stenman Jakob, Tho Ho

Abstract excerpt

The missense mutation EGFR L858R implies increased sensitivity to EGFR tyrosine kinase inhibitor (TKIs) therapy, despite a significant non-response rate. Currently, detection of EGFR L858R mutation is mostly DNA based, therefore, the allele-specific expression level of the mutated gene and its clinical relevance is hidden. Based on the extendable blocking probes and hot-start protocol for reverse transcription,...

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PMID: 35780814
DOI: 10.1016/j.ab.2022.114799

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