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Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures

1 Jun 2022

Abstract excerpt

Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expansion and maintenance of AT2s. The culture conditions are scalable and compatible with high-throughput chemical and...

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PMID: 35712012
DOI: 10.1016/j.xpro.2022.101447

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