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Protocol for Cloning Epithelial Stem Cell Variants from Human Lung

9 Jul 2020

Abstract excerpt

The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of these stem cell variants, which in general assist in analyses of stem cell heterogeneity, genome editing, drug screening, and...

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PMID: 33015646
DOI: 10.1016/j.xpro.2020.100063

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