Article
SEB genotyping: SmartAmp-Eprimer binary code genotyping for complex, highly variable targets applied to HBV.
BMC infectious diseases - 3 Jun 2022
Delobel Diane, Furutani Yutaka, Nagoshi Sumiko, Tsubota Akihito, Miyasaka Akio, Watashi Koichi, Wakita Takaji, Matsuura Tomokazu, Usui Kengo
Abstract excerpt
BACKGROUND: SmartAmp-Eprimer Binary code (SEB) Genotyping is a novel isothermal amplification method for rapid genotyping of any variable target of interest. METHODS: After in silico alignment of a large number of sequences and computational analysis to determine the smallest number of regions to be targeted by SEB Genotyping, SmartAmp primer sets were designed to obtain a binary code of On/Off fluorescence...
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