Article
DNA methylation can alter CRISPR/Cas9 editing frequency and DNA repair outcome in a target-specific manner.
The New phytologist - 1 Sept 2022
Přibylová Adéla, Fischer Lukáš, Pyott Douglas E, Bassett Andrew, Molnar Attila
Abstract excerpt
The impact of epigenetic modifications on the efficacy of CRISPR/Cas9-mediated double-stranded DNA breaks and subsequent DNA repair is poorly understood, especially in plants. In this study, we investigated the effect of the level of cytosine methylation on the outcome of CRISPR/Cas9-induced mutations at multiple Cas9 target sites in Nicotiana benthamiana leaf cells using next-generation sequencing. We found that...
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