Article
Improving transcriptional activity of human cytomegalovirus major immediate-early promoter by mutating NF-κB binding sites.
Protein expression and purification - 1 Feb 2018
Wang Danyang, Dai Wei, Wu Jian, Wang Jinke
Abstract excerpt
Many mammalian gene expression vectors express the transferred genes under the control of the cytomegalovirus (CMV) major immediate-early promoter (MIEP). The human MIEP has been known as the strongest promoter in mammalian cells and utilized widely in mammalian expression systems. There are four NF-κB binding sites (named as κBs) in the human MIEP. In this study, we have constructed multiple mutated MIEPs by...
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