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Article

High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease.

Plant cell reports - 1 Jul 2016

Eid Ayman, Ali Zahir, Mahfouz Magdy M

Abstract excerpt

KEY MESSAGE: The use of a meiosis I-specific promoter increased the efficiency of targeted mutagenesis and will facilitate the manipulation of homologous recombination. The CRISPR/Cas9 system has been harnessed for targeted engineering of eukaryotic genomes, including plants; however, CRISPR/Cas9 efficiency varies considerably in different plant tissues and species. In Arabidopsis, the generation of homozygous or...

Read the complete abstract on PubMed

Topics

Arabidopsis
Arabidopsis Proteins
Base Sequence
CRISPR-Cas Systems
Endonucleases
Gene Editing
Genetic Engineering

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Homologous Recombination

Homozygote

Meiosis

Models, Genetic

Mutagenesis, Site-Directed

Mutation

Plants, Genetically Modified

Promoter Regions, Genetic

Reproducibility of Results

Source

Identifiers

PMID: 27236699
DOI: 10.1007/s00299-016-2000-4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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