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Quantitative PCR-Based Method to Assess Cell-Free DNA Quality, Adjust Input Mass, and Improve Next-Generation Sequencing Assay Performance.

The Journal of molecular diagnostics : JMD - 1 Jun 2022

Saelee Seng Lor, Lovejoy Alexander F, Hinzmann Bernd, Mayol Katrina, Huynh Samantha, Harrell Amy, Lefkowitz Josh, Deodhar Niharika, Garcia-Montoya Gladys, Yaung Stephanie J, Klass Daniel M

Abstract excerpt

Cell-free (cf)DNA-based testing has undergone increasingly wide adoption, including assays for the detection of circulating tumor DNA. Due to nucleosome protection, cfDNA has a distinctive fragment size of 160 to 180 bp. However, cfDNA can be contaminated with high molecular weight genomic DNA from blood cells released in plasma during sample collection. Such contamination can lead to decreased sensitivity or...

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PMID: 35364322
DOI: 10.1016/j.jmoldx.2022.02.005

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