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Detection of Circulating Tumor DNA in Plasma Using Targeted Sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Sun Xue, Liu Xingyu, Zhao Ying, Tian Geng, Wang Weiwei

Abstract excerpt

Cell-free DNA (cfDNA) is the degradation product of extracellular DNA. Circulating tumor DNA (ctDNA), as a fraction of cfDNA, comes from tumor cells and contains variations, including mutation, deletion, insertion, rearrangement, copy number variation, and methylation. Therefore, biomarkers identified in ctDNA show promising clinical applications in early diagnosis, recurrence monitoring, and conducting...

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PMID: 37450110
DOI: 10.1007/978-1-0716-3346-5_3

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