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CRISPR activation and interference screens decode stimulation responses in primary human T cells

3 Feb 2022

Abstract excerpt

Regulation of cytokine production in stimulated T cells can be disrupted in autoimmunity, immunodeficiencies, and cancer. Systematic discovery of stimulation-dependent cytokine regulators requires both loss-of-function and gain-of-function studies, which have been challenging in primary human cells. We now report genome-wide CRISPR activation (CRISPRa) and interference (CRISPRi) screens in primary human T cells...

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PMID: 35113687
DOI: 10.1126/science.abj4008

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