Article
Altered specificities of genetically engineered alpha 1 antitrypsin variants.
Protein engineering - 1 Jan 2000
Jallat S, Carvallo D, Tessier L H, Roecklin D, Roitsch C, Ogushi F, Crystal R G, Courtney M
Abstract excerpt
Seven active site variants of human alpha 1-antitrypsin (alpha 1AT) were produced in Escherichia coli following site-specific mutagenesis of the alpha 1AT complementary DNA. alpha 1AT (Ala358), alpha 1AT (Ile358) and alpha 1AT (Val358) were efficient inhibitors of both neutrophil and pancreatic e...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cathepsin G
- Cathepsins
- Genetic Variation
- Humans
- In Vitro Techniques
- Molecular Sequence Data
- Pancreatic Elastase
- Protein Engineering
- Serine Endopeptidases
- Thrombin
- alpha 1-Antitrypsin