Article
Use of site-directed mutagenesis to obtain isomorphous heavy-atom derivatives for protein crystallography: cysteine-containing mutants of phage T4 lysozyme.
Protein engineering - 1 Jan 2000
Sun D P, Alber T, Bell J A, Weaver L H, Matthews B W
Abstract excerpt
Five different cysteine-containing mutants of the lysozyme from bacteriophage T4 were used to explore the feasibility of using site-directed mutagenesis to generate isomorphous heavy-atom derivatives for protein crystallography. Cysteines 54 and 97, present in wild-type lysozyme, can be readily r...
Topics
- Amino Acid Sequence
- Binding Sites
- Cysteine
- Mercury
- Molecular Sequence Data
- Molecular Structure
- Muramidase
- Mutation
- Protein Engineering
- T-Phages
- X-Ray Diffraction