Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme.

Protein science : a publication of the Protein Society - 1 Oct 1998

Jucovic M, Poteete A R

Abstract excerpt

Model-free approaches (random mutagenesis, DNA shuffling) in combination with more "rational," three-dimensional information-guided randomization have been used for directed evolution of lysozyme activity in a defective T4 lysozyme mutant. A specialized lysozyme cloning vector phage, derived from phage lambda, depends upon T4 lysozyme function for its ability to form plaques. The substitution W138P in T4 lysozyme...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Codon
Share to DNA, Viral
Share to Genetic Vectors

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Models, Molecular

Share to Mutagenesis

Share to Mutation

Share to Polymerase Chain Reaction

Share to Recombination, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 9792108
DOI: 10.1002/pro.5560071018
PMCID: PMC2143835

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme.PMID 9792108Read ↗

Protein salvage by directed evolution. Functional restoration of a defective lysozyme mutant.Semantic proximity Read ↗

Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations.Semantic proximity Read ↗

Lessons from the lysozyme of phage T4.Semantic proximity Read ↗

Directed evolution studies with combinatorial libraries of T4 lysozyme mutants.Semantic proximity Read ↗

Structures of randomly generated mutants of T4 lysozyme show that protein stability can be enhanced by relaxation of strain and by improved hydrogen bonding via bound solvent.Semantic proximity Read ↗

Construction of a screening system for selecting lysozyme mutants unable to form a stable structure from random mutants.Semantic proximity Read ↗

Systematic mutation of bacteriophage T4 lysozyme.Semantic proximity Read ↗

Crystal structures of a T4-lysozyme duplication-extension mutant demonstrate that the highly conserved beta-sheet region has low intrinsic folding propensity.Semantic proximity Read ↗

Use of site-directed mutagenesis to obtain isomorphous heavy-atom derivatives for protein crystallography: cysteine-containing mutants of phage T4 lysozyme.Semantic proximity Read ↗

Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position.Semantic proximity Read ↗

Structure-based design of a lysozyme with altered catalytic activity.Semantic proximity Read ↗

Thermodynamic and structural compensation in "size-switch" core repacking variants of bacteriophage T4 lysozyme.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.