Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme.

doi:10.1002/pro.5560071018

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Article

Delineation of an evolutionary salvage pathway by compensatory mutations of a defective lysozyme.

Protein science : a publication of the Protein Society - 1 Oct 1998

Jucovic M, Poteete A R

Abstract excerpt

Model-free approaches (random mutagenesis, DNA shuffling) in combination with more "rational," three-dimensional information-guided randomization have been used for directed evolution of lysozyme activity in a defective T4 lysozyme mutant. A specialized lysozyme cloning vector phage, derived from...

Topics

Bacteriophage T4
Bacteriophage lambda
Codon
DNA, Viral
Evolution, Molecular
Genetic Vectors
Models, Molecular
Muramidase
Mutagenesis
Mutation
Polymerase Chain Reaction
Recombination, Genetic
Suppression, Genetic

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