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Article

The role of cysteine oxidation in the thermal inactivation of T4 lysozyme.

Protein engineering - 1 Jan 2000

Perry L J, Wetzel R

Abstract excerpt

Wild-type T4 lysozyme contains unpaired cysteine residues at positions 54 and 97. To investigate the role these residues play in the thermal inactivation of the wild-type, we constructed a double mutant with these cysteines replaced with valine and serine. This molecule, T4 lysozyme (C54V/C97S), is more stable than the wild-type to inactivation at 70 degrees C at pH 6.5 and 8.0. Guanidine hydrochloride...

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PMID: 3507692
DOI: 10.1093/protein/1.2.101

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SDS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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