Article
Juggling with fluorescent proteins: Spectrum and structural changes of the mCardinal2 variants.
Biochemical and biophysical research communications - 19 Feb 2022
Kim Tae-Yeon, Yoon Tae-Sung, Kang Sunghyun, Afzal Muhammad
Abstract excerpt
mCardinal2 is a red fluorescent protein developed through the designs of mKate, mNeptune and mCardinal. Fluorescence spectrums of mCardinal2 and its five mutants (T143C, T143G, C158A, C158D and M160E) were measured with their quantum yields. C158A and C158D increased brightness with slight changes in fluorescence spectrums while T143C, T143G and M160E decreased brightness with blue shift in fluorescence...
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