Article
Coordination of -1 programmed ribosomal frameshifting by transcript and nascent chain features revealed by deep mutational scanning.
Nucleic acids research - 16 Dec 2021
Carmody Patrick J, Zimmer Matthew H, Kuntz Charles P, Harrington Haley R, Duckworth Kate E, Penn Wesley D, Mukhopadhyay Suchetana, Miller Thomas F, Schlebach Jonathan P
Abstract excerpt
Programmed ribosomal frameshifting (PRF) is a translational recoding mechanism that enables the synthesis of multiple polypeptides from a single transcript. During translation of the alphavirus structural polyprotein, the efficiency of -1PRF is coordinated by a 'slippery' sequence in the transcript, an adjacent RNA stem-loop, and a conformational transition in the nascent polypeptide chain. To characterize each...
Topics
- Alphavirus
- Frameshifting, Ribosomal
- HEK293 Cells
- Humans
- Kinetics
- Molecular Dynamics Simulation
- Mutation
- Nucleic Acid Conformation
- Polyproteins
- Protein Biosynthesis
- RNA, Messenger
- RNA, Transfer
- RNA, Viral