Article
Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization.
Nature communications - 18 Nov 2021
Friedrich Nikolas, Stiegeler Emanuel, Glögl Matthias, Lemmin Thomas, Hansen Simon, Kadelka Claus, Wu Yufan, Ernst Patrick, Maliqi Liridona, Foulkes Caio, Morin Mylène, Eroglu Mustafa, Liechti Thomas, Ivan Branislav, Reinberg Thomas, Schaefer Jonas V, Karakus Umut, Ursprung Stephan, Mann Axel, Rusert Peter, Kouyos Roger D, Robinson John A, Günthard Huldrych F, Plückthun Andreas, Trkola Alexandra
Abstract excerpt
The V3 loop of the HIV-1 envelope (Env) protein elicits a vigorous, but largely non-neutralizing antibody response directed to the V3-crown, whereas rare broadly neutralizing antibodies (bnAbs) target the V3-base. Challenging this view, we present V3-crown directed broadly neutralizing Designed Ankyrin Repeat Proteins (bnDs) matching the breadth of V3-base bnAbs. While most bnAbs target prefusion Env, V3-crown...
Topics
- Antibodies, Neutralizing
- Cell Line, Tumor
- Epitopes
- HEK293 Cells
- HIV Antibodies
- HIV-1
- Humans
- Immunoglobulin G
- Molecular Docking Simulation