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Article

Repairing TALEN-mediated double-strand break by microhomology-mediated recombination in tobacco plastids generates abundant subgenomic DNA.

Plant science : an international journal of experimental plant biology - 1 Dec 2021

Huang Chih-Hao, Liu Yu-Chang, Shen Jia-Yi, Lu Fu-I, Shaw Shyh-Yu, Huang Hao-Jen, Chang Ching-Chun

Abstract excerpt

Transcription activator-like effector nuclease (TALEN) technology has been widely used to edit nuclear genomes in plants but rarely for editing organellar genomes. In addition, ciprofloxacin, commonly used to cause the double-strand break of organellar DNA for studying the repair mechanism in plants, confers no organellar selectivity and site-specificity. To demonstrate the feasibility of TALEN-mediated...

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PMID: 34763881
DOI: 10.1016/j.plantsci.2021.111028

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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