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Reverse genetics system for Tick-borne encephalitis virus using Circular Polymerase Extension Reaction

1 Jun 2025

Abstract excerpt

Tick-borne encephalitis virus (TBEV) belongs to the Family Flaviviridae, Genus Orthoflavivirus, and causes severe neurological diseases in humans. The reverse genetics system is a basic tool for viral research; however, cloning the genome of orthoflavivirus using bacterial plasmids is difficult because of their toxicity to Escherichia coli. Polymerase chain reaction-based Circular Polymerase Extension Reaction...

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Insect and Pesticide Research
Mosquito-borne diseases and control
Vector-borne infectious diseases

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 40460901
DOI: 10.1016/j.meegid.2025.105776

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