Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutation of βGln114 to Ala Alters the Stabilities of Allosteric States in Tryptophan Synthase Catalysis.

Biochemistry - 26 Oct 2021

Ghosh Rittik K, Hilario Eduardo, Liu Viktoriia, Wang Yangyang, Niks Dimitri, Holmes Jacob B, Sakhrani Varun V, Mueller Leonard J, Dunn Michael F

Abstract excerpt

The tryptophan synthase (TS) bienzyme complexes found in bacteria, yeasts, and molds are pyridoxal 5'-phosphate (PLP)-requiring enzymes that synthesize l-Trp. In the TS catalytic cycle, switching between the open and closed states of the α- and β-subunits via allosteric interactions is key to the efficient conversion of 3-indole-d-glycerol-3'-phosphate and l-Ser to l-Trp. In this process, the roles played by...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Allosteric Regulation
Share to Bacterial Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Biocatalysis

Share to Kinetics

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Salmonella typhimurium

Share to Tryptophan Synthase

Source

Open in PubMed Open full text

Identifiers

PMID: 34595921
DOI: 10.1021/acs.biochem.1c00383
PMCID: PMC9122093

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutation of βGln114 to Ala Alters the Stabilities of Allosteric States in Tryptophan Synthase Catalysis.PMID 34595921Read ↗

A Molecular Dynamics Simulation Study of the Effects of βGln114 Mutation on the Dynamic Behavior of the Catalytic Site of the Tryptophan Synthase.Semantic proximity Read ↗

Crystal structures of a mutant (betaK87T) tryptophan synthase alpha2beta2 complex with ligands bound to the active sites of the alpha- and beta-subunits reveal ligand-induced conformational changes.Semantic proximity Read ↗

On the role of alphaThr183 in the allosteric regulation and catalytic mechanism of tryptophan synthase.Semantic proximity Read ↗

Allosteric communication of tryptophan synthase. Functional and regulatory properties of the beta S178P mutant.Semantic proximity Read ↗

Crystal structure of the beta Ser178--> Pro mutant of tryptophan synthase. A "knock-out" allosteric enzyme.Semantic proximity Read ↗

The tryptophan synthase multienzyme complex: exploring structure-function relationships with X-ray crystallography and mutagenesis.Semantic proximity Read ↗

The tryptophan synthase alpha 2 beta 2 complex: kinetic studies with a mutant enzyme (beta K87T) to provide evidence for allosteric activation by an aminoacrylate intermediate.Semantic proximity Read ↗

Thermal repair of tryptophan synthase mutations in a regulatory intersubunit salt bridge. Evidence from arrhenius plots, absorption spectra, and primary kinetic isotope effects.Semantic proximity Read ↗

Genetic and biochemical characterization of the trpB8 mutation of Escherichia coli tryptophan synthase. An amino acid switch at the sharp turn of the trypsin-sensitive "hinge" region diminishes substrate binding and alters solubility.Semantic proximity Read ↗

Structural basis for the impaired channeling and allosteric inter-subunit communication in the beta A169L/beta C170W mutant of tryptophan synthase.Semantic proximity Read ↗

Threonine 183 and adjacent flexible loop residues in the tryptophan synthase alpha subunit have critical roles in modulating the enzymatic activities of the beta subunit in the alpha 2 beta 2 complex.Semantic proximity Read ↗

Serine modulates substrate channeling in tryptophan synthase. A novel intersubunit triggering mechanism.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.