Article
Serine modulates substrate channeling in tryptophan synthase. A novel intersubunit triggering mechanism.
The Journal of biological chemistry - 5 May 1991
Anderson K S, Miles E W, Johnson K A
Abstract excerpt
Tryptophan synthase, an alpha 2 beta 2 complex, is a classic example of an enzyme that is thought to "channel" a metabolic intermediate (indole) from the active site of the alpha subunit to the active site of the beta subunit. We now examine the kinetics of substrate channeling by tryptophan synthase directly by chemical quench-flow and stopped-flow methods. The conversion of indole-3-glycerol phosphate (IGP) to...
Topics
- Binding Sites
- Chromatography, High Pressure Liquid
- Computer Simulation
- Fluorescence
- Glycerophosphates
- Indoles
- Kinetics
- Molecular Structure
- Mutation
- Protein Conformation
- Salmonella typhimurium