Article
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli.
Communications biology - 25 Mar 2020
Shimojo Masaru, Amikura Kazuaki, Masuda Keiko, Kanamori Takashi, Ueda Takuya, Shimizu Yoshihiro
Abstract excerpt
In vitro reconstitution is a powerful tool for investigating ribosome functions and biogenesis, as well as discovering new ribosomal features. In this study, we integrated all of the processes required for Escherichia coli small ribosomal subunit assembly. In our method, termed fully Recombinant-based integrated Synthesis, Assembly, and Translation (R-iSAT), assembly and evaluation of the small ribosomal subunits...
Topics
- Bacterial Proteins
- Escherichia coli
- Mutation
- RNA, Bacterial
- RNA, Ribosomal, 16S
- Ribosomal Proteins
- Ribosome Subunits, Small, Bacterial
- Transcription, Genetic