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Article

An Improved Tat/Rev Induced Limiting Dilution Assay With Enhanced Sensitivity and Breadth of Detection.

Frontiers in immunology - 1 Jan 2021

Mehta Kavita, Gohil Yuvrajsinh, Mishra Swarnima, D'silva Anish, Amanullah Afzal, Selvam Deepak, Pargain Neelam, Nala Narendra, Sanjeeva G N, Ranga Udaykumar

Abstract excerpt

Tat/Rev Induced Limiting Dilution Assay (TILDA) is instrumental in estimating the size of latent reservoirs of HIV-1. Here, we report an optimized TILDA containing a broader detection range compared to the reported methods and high sensitivity. Giving priority to sequence conservation, we positioned the two forward primers and the probe in exon-1 of HIV-1. The reverse primers are positioned in highly conserved...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to rev Gene Products, Human Immunodeficiency Virus

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Share to tat Gene Products, Human Immunodeficiency Virus

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Source

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Identifiers

PMID: 34421920
DOI: 10.3389/fimmu.2021.715644
PMCID: PMC8375296

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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