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Article

A Novel PCR Assay for Quantification of HIV-1 RNA

27 Mar 2013

Abstract excerpt

Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This...

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PMID: 23536672
DOI: 10.1128/jvi.00006-13

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