Article
Cross-platform comparison of next-generation sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for detecting KRAS/NRAS/BRAF/PIK3CA mutations in cfDNA from metastatic colorectal cancer patients.
Journal of clinical laboratory analysis - 1 Sept 2021
Xu Xiaojing, Huang Fei, Cao Minlu, Chen Xinning, Wang Hao, Jiang Huiqin, Yu Yiyi, Shen Minna, Yang Yihui, Wang Beili, Liu Tianshu, Guo Wei
Abstract excerpt
BACKGROUND: Examining tumor KRAS/NRAS/BRAF/PIK3CA status in metastatic colorectal cancer (mCRC) is essential for treatment selection and prognosis evaluation. Cell-free DNA (cfDNA) in plasma is a feasible source for tumor gene analysis. METHODS: In this study, we recruited mCRC patients and analyzed their KRAS/NRAS/BRAF/PIK3CA status in cfDNA using two platforms, next-generation sequencing (NGS) and...
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