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Article

A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis.

Plant & cell physiology - 10 Dec 2021

Kurokawa Shuta, Rahman Hafizur, Yamanaka Naoshi, Ishizaki Chisato, Islam Shaikhul, Aiso Tsuyoshi, Hirata Shunya, Yamamoto Mayuka, Kobayashi Kappei, Kaya Hidetaka

Abstract excerpt

The CRISPR/Cas9 system is now commonly employed for genome editing in various plants such as Arabidopsis, rice and tobacco. In general, in genome editing of the Arabidopsis genome, the SpCas9 and guide RNA genes are introduced into the genome by the floral dip method. Mutations induced in the target sequence by SpCas9 are confirmed after selecting transformants by screening the T1 seed population. The advantage...

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PMID: 34347875
DOI: 10.1093/pcp/pcab123

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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