Article
Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress.
The Plant journal : for cell and molecular biology - 1 Jan 2018
LeBlanc Chantal, Zhang Fei, Mendez Josefina, Lozano Yamile, Chatpar Krishna, Irish Vivian F, Jacob Yannick
Abstract excerpt
The CRISPR/Cas9 system has greatly improved our ability to engineer targeted mutations in eukaryotic genomes. While CRISPR/Cas9 appears to work universally, the efficiency of targeted mutagenesis and the adverse generation of off-target mutations vary greatly between different organisms. In this study, we report that Arabidopsis plants subjected to heat stress at 37°C show much higher frequencies of...
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