Article
Continual improvement of CRISPR-induced multiplex mutagenesis in Arabidopsis.
The Plant journal : for cell and molecular biology - 1 Jul 2024
Develtere Ward, Decaestecker Ward, Rombaut Debbie, Anders Chantal, Clicque Elke, Vuylsteke Marnik, Jacobs Thomas B
Abstract excerpt
CRISPR/Cas9 is currently the most powerful tool to generate mutations in plant genomes and more efficient tools are needed as the scale of experiments increases. In the model plant Arabidopsis, the choice of the promoter driving Cas9 expression is critical to generate germline mutations. Several optimal promoters have been reported. However, it is unclear which promoter is ideal as they have not been thoroughly...
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