Article
A Method to Reduce off-Targets in CRISPR/Cas9 System in Plants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Movahedi Ali, Hajiahmadi Zahra, Wei Hui, Yang Liming, Ruan Honghua, Zhuge Qiang
Abstract excerpt
One of the strategies to reduce the off-target mutations in CRISPR/Cas9 system is to use the temperature-independent gene transformation method. Mesoporous silica nanoparticles (MSNs)-gene delivery system is temperature-independent; thus, it can transfer the interesting plasmid (pDNA) to the target plant at different temperatures, including 37 °C. Due to the high activity of SpCas9 at 37 °C compared to lower...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.