Article
PolyG-DS: An ultrasensitive polyguanine tract-profiling method to detect clonal expansions and trace cell lineage.
Proceedings of the National Academy of Sciences of the United States of America - 3 Aug 2021
Zhang Yuezheng, Kohrn Brendan F, Yang Ming, Nachmanson Daniela, Soong T Rinda, Lee I-Hsiu, Tao Yong, Clevers Hans, Swisher Elizabeth M, Brentnall Teresa A, Loeb Lawrence A, Kennedy Scott R, Salk Jesse J, Naxerova Kamila, Risques Rosa Ana
Abstract excerpt
Polyguanine tracts (PolyGs) are short guanine homopolymer repeats that are prone to accumulating mutations when cells divide. This feature makes them especially suitable for cell lineage tracing, which has been exploited to detect and characterize precancerous and cancerous somatic evolution. PolyG genotyping, however, is challenging because of the inherent biochemical difficulties in amplifying and sequencing...
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