Article
A CRISPR/Cas9-mediated gene knockout system in Aspergillus luchuensis mut. kawachii.
Bioscience, biotechnology, and biochemistry - 1 Oct 2020
Kadooka Chihiro, Yamaguchi Masaaki, Okutsu Kayu, Yoshizaki Yumiko, Takamine Kazunori, Katayama Takuya, Maruyama Jun-Ichi, Tamaki Hisanori, Futagami Taiki
Abstract excerpt
We developed an approach to genome editing of the white koji fungus, Aspergillus luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Co-transformation of AMA1-based Cas9 and gRNA expression plasmids achieved efficient gene knockout in A. kawachii. The plasmids were easily lost when selective pressure was removed, allowing for successive rounds of genome...
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