Article
Highly efficient CRISPR/Cas9-mediated targeted mutagenesis of multiple genes in Populus.
Yi chuan = Hereditas - 1 Oct 2015
Liu Ting-ting, Fan Di, Ran Ling-yu, Jiang Yuan-zhong, Liu Rui, Luo Ke-ming
Abstract excerpt
The typeⅡCRISPR/Cas9 system (Clustered regularly interspaced short palindromic repeats /CRISPR-associated 9) has been widely used in bacteria, yeast, animals and plants as a targeted genome editing technique. In previous work, we have successfully knocked out the endogenous phytoene dehydrogenase (PDS) gene in Populus tomentosa Carr. using this system. To study the effect of target design on the efficiency of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
