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Article

Increased mutation efficiency of CRISPR/Cas9 genome editing in banana by optimized construct.

PeerJ - 1 Jan 2022

Zhang Sen, Wu Shaoping, Hu Chunhua, Yang Qiaosong, Dong Tao, Sheng Ou, Deng Guiming, He Weidi, Dou Tongxin, Li Chunyu, Sun Chenkang, Yi Ganjun, Bi Fangcheng

Abstract excerpt

The CRISPR/Cas9-mediated genome editing system has been used extensively to engineer targeted mutations in a wide variety of species. Its application in banana, however, has been hindered because of the species' triploid nature and low genome editing efficiency. This has delayed the development of a DNA-free genome editing approach. In this study, we reported that the endogenous U6 promoter and banana...

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PMID: 35036088
DOI: 10.7717/peerj.12664
PMCID: PMC8742547

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