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Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Ito Kyohei, Xu Lejia, Ito Genta, Tomita Taisuke

Abstract excerpt

Recent studies revealed that leucine-rich repeat kinase 2 (LRRK2) phosphorylates several Rab proteins under physiological conditions. Mutations linked with familial Parkinson's disease cause an abnormal increase in the Rab phosphorylation, which has not been elucidated in an in vitro kinase assays where artificial peptide substrates are often used. Here, we provide protocols for detecting the LRRK2 activity in...

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PMID: 34043192
DOI: 10.1007/978-1-0716-1495-2_6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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