Article
Imaging-based live cell yeast screen identifies novel factors involved in peroxisome assembly.
Journal of proteome research - 1 Jan 2009
Wolinski Heimo, Petrovic Uros, Mattiazzi Mojca, Petschnigg Julia, Heise Bettina, Natter Klaus, Kohlwein Sepp D
Abstract excerpt
We describe an imaging-based method in intact cells to systematically screen yeast mutant libraries for abnormal morphology and distribution of fluorescently labeled subcellular structures. In this study, chromosomally expressed green fluorescent protein (GFP) fused to the peroxisomal targeting sequence 1, consisting of serine-lysine-leucine, was introduced into 4740 viable yeast deletion mutants using a modified...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
