Article
Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Gunkel Manuel, Eberle Jan Philipp, Erfle Holger
Abstract excerpt
Applying the right acquisition method in a fluorescence imaging-based screening context is of great importance to obtain an appropriate readout and to select the right scale of the screen. In order to save imaging time and data, we have developed routines for multiscale targeted imaging, providing both a broad overview of a sample and additional in-depth information for targets of interest identified within the...
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