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Article

Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells

1 May 2021

Abstract excerpt

Human periimplantation development requires the transformation of the naive pluripotent epiblast into a polarized epithelium. Lumenogenesis plays a critical role in this process, as the epiblast undergoes rosette formation and lumen expansion to form the amniotic cavity. Here, we present a high-throughput in vitro model for epiblast morphogenesis. We established a microfluidic workflow to encapsulate human...

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PMID: 33979603
DOI: 10.1016/j.stemcr.2021.04.009

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