Simultaneous gene editing of three homoeoalleles in self-incompatible allohexaploid grasses.

Abstract excerpt

Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been widely used for precise gene editing in plants. However, simultaneous gene editing of multiple homoeoalleles remains challenging, especially in self-incompatible polyploid plants. Here, we simultaneously introduced targeted mutations in all three homoeoalleles of two genes in the self-incompatible...

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• Base Sequence
• CRISPR-Cas Systems
• Gene Editing
• Heat-Shock Response
• Mutagenesis