Article
Allele-aware chromosome-level genome assembly and efficient transgene-free genome editing for the autotetraploid cultivated alfalfa.
Nature communications - 19 May 2020
Chen Haitao, Zeng Yan, Yang Yongzhi, Huang Lingli, Tang Bolin, Zhang He, Hao Fei, Liu Wei, Li Youhan, Liu Yanbin, Zhang Xiaoshuang, Zhang Ru, Zhang Yesheng, Li Yongxin, Wang Kun, He Hua, Wang Zhongkai, Fan Guangyi, Yang Hui, Bao Aike, Shang Zhanhuan, Chen Jianghua, Wang Wen, Qiu Qiang
Abstract excerpt
Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy and self-incompatibility. Here, we generate an allele-aware chromosome-level genome assembly for the cultivated alfalfa consisting of 32 allelic chromosomes by...
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