Article
Implementing the CRISPR/Cas9 Technology in Eucalyptus Hairy Roots Using Wood-Related Genes.
International journal of molecular sciences - 12 May 2020
Dai Ying, Hu Guojian, Dupas Annabelle, Medina Luciano, Blandels Nils, Clemente Hélène San, Ladouce Nathalie, Badawi Myriam, Hernandez-Raquet Guillermina, Mounet Fabien, Grima-Pettenati Jacqueline, Cassan-Wang Hua
Abstract excerpt
Eucalypts are the most planted hardwoods worldwide. The availability of the Eucalyptus grandis genome highlighted many genes awaiting functional characterization, lagging behind because of the lack of efficient genetic transformation protocols. In order to efficiently generate knock-out mutants to study the function of eucalypts genes, we implemented the powerful CRISPR/Cas9 gene editing technology with the hairy...
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