Article
Rationally Designed Base Editors for Precise Editing of the Sickle Cell Disease Mutation.
The CRISPR journal - 1 Apr 2021
Chu S Haihua, Packer Michael, Rees Holly, Lam Dieter, Yu Yi, Marshall Jeffrey, Cheng Lo-I, Lam Daisy, Olins Jenny, Ran Fei Ann, Liquori Alexander, Gantzer Bob, Decker Jeremy, Born David, Barrera Luis, Hartigan Adam, Gaudelli Nicole, Ciaramella Giuseppe, Slaymaker Ian M
Abstract excerpt
Base editors are fusions of a deaminase and CRISPR-Cas ribonucleoprotein that allow programmable installment of transition mutations without double-strand DNA break intermediates. The breadth of potential base editing targets is frequently limited by the requirement of a suitably positioned Cas9 protospacer adjacent motif. To address this, we used structures of Cas9 and TadA to design a set of inlaid base editors...
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