Article
A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria.
Proceedings of the National Academy of Sciences of the United States of America - 16 Mar 2021
Dik David A, Zhang Nan, Sturgell Emily J, Sanchez Brittany B, Chen Jason S, Webb Bill, Vanderpool Kimberly G, Schultz Peter G
Abstract excerpt
Gram-positive bacteria assemble a multilayered cell wall that provides tensile strength to the cell. The cell wall is composed of glycan strands cross-linked by nonribosomally synthesized peptide stems. Herein, we modify the peptide stems of the Gram-positive bacterium Bacillus subtilis with noncanonical electrophilic d-amino acids, which when in proximity to adjacent stem peptides form novel covalent...
Topics
- Amino Acids
- Bacillus subtilis
- Cell Wall
- Gram-Negative Bacteria
- Gram-Positive Rods
- Peptidoglycan
- Peptidyl Transferases
- Phenotype