Article
Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica.
Science China. Life sciences - 1 Dec 2021
Bai Qiuyan, Cheng Shuai, Zhang Jinlai, Li Mengxu, Cao Yingxiu, Yuan Yingjin
Abstract excerpt
Genomic variants libraries are conducive to obtain dominant strains with desirable phenotypic traits. The non-homologous end joining (NHEJ), which enables foreign DNA fragments to be randomly integrated into different chromosomal sites, shows prominent capability in genomic libraries construction. In this study, we established an efficient NHEJ-mediated genomic library technology in Yarrowia lipolytica through...
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