Article
Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples.
BMC cancer - 4 Feb 2021
Fu Chunxiao, Marczyk Michal, Samuels Michael, Trevarton Alexander J, Qu Jiaxin, Lau Rosanna, Du Lili, Pappas Todd, Sinn Bruno V, Gould Rebekah E, Pusztai Lajos, Hatzis Christos, Symmans W Fraser
Abstract excerpt
BACKGROUND: Our objective was to assess whether modifications to a customized targeted RNA sequencing (RNAseq) assay to include unique molecular identifiers (UMIs) that collapse read counts to their source mRNA counts would improve quantification of transcripts from formalin-fixed paraffin-embedded (FFPE) tumor tissue samples. The assay (SET4) includes signatures that measure hormone receptor and PI3-kinase...
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