Computation-guided optimization of split protein systems.

Abstract excerpt

Splitting bioactive proteins into conditionally reconstituting fragments is a powerful strategy for building tools to study and control biological systems. However, split proteins often exhibit a high propensity to reconstitute, even without the conditional trigger, limiting their utility. Current approaches for tuning reconstitution propensity are laborious, context-specific or often ineffective. Here, we report...

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• Endopeptidases
• Luminescent Proteins
• Molecular Probes
• Protein Engineering
• Protein Multimerization